Analysis of proteomic changes induced upon cellular differentiation of the human intestinal cell line Caco-2
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چکیده
منابع مشابه
Fatty acid esterification during differentiation of the human intestinal cell line Caco-2.
The Caco-2 human intestinal cell line was used to examine fatty acid esterification during development of the enterocytic phenotype. Acyl-CoA synthetase activity increased approximately 40%, and the incorporation of palmitic acid into triacylglycerol relative to phosphatidylcholine increased nearly 2-fold during Caco-2 differentiation. A rate-limiting enzyme activity in the glycerol 3-phosphate...
متن کاملIncreased expression of apolipoprotein genes accompanies differentiation in the intestinal cell line Caco-2.
We have analyzed determinants of the synthesis and secretion of apolipoproteins including mRNA for apolipoproteins, in the human colon carcinoma cell line Caco-2 during differentiation in continuous culture. Significant increases in both cellular and secreted apolipoprotein A-I were observed early in the differentiation process. Increases in apolipoprotein B were limited to secreted protein and...
متن کاملCellular uptake and efflux of the tea flavonoid (-)epicatechin-3-gallate in the human intestinal cell line Caco-2.
(-)-Epicatechin gallate (ECG) is one of the flavonoids in green tea, which has been demonstrated to have cancer-preventive properties in many model systems. However, the extent and mechanisms of accumulation of these flavonoids in cells is unknown. The objectives of this study were to determine the accumulation of ECG by the intestinal epithelial cell Caco-2 and to characterize the transport me...
متن کاملGlycosyltransferase Changes upon Differentiation of CaCo-2 Human Colonie Adenocarcinoma Cells1
The spontaneous differentiation of CaCo-2 human colonie adenocarcinuma cells to enterocytes in culture is associated with a decrease in polylactosaminoglycans, particularly those attached to the lysosomal membrane glycoprotein h-lamp-1 (Youakim et al., Cancer Res., 49:68896895, 1989). To elucidate the biosynthetic mechanisms leading to these alterations we have compared glycosyltransferase acti...
متن کاملGlycosyltransferase changes upon differentiation of CaCo-2 human colonic adenocarcinoma cells.
The spontaneous differentiation of CaCo-2 human colonic adenocarcinoma cells to enterocytes in culture is associated with a decrease in polylactosaminoglycans, particularly those attached to the lysosomal membrane glycoprotein h-lamp-1 (Youakim et al., Cancer Res., 49:6889-6895, 1989). To elucidate the biosynthetic mechanisms leading to these alterations we have compared glycosyltransferase act...
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ژورنال
عنوان ژورنال: Development, Growth & Differentiation
سال: 2011
ISSN: 0012-1592
DOI: 10.1111/j.1440-169x.2011.01258.x